Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Naca and Lrp6 form part of a common genetic pathway regulating bone mass. Femurs of female mice were analyzed by μCT at 3 months of age. BV/TV, bone volume/tissue volume. * p < 0.05, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: The osteoanabolic response to iPTH is inhibited in Naca / Lrp6 compound heterozygotes. Four-month-old female mice were treated once daily, 5 days/week for 1 month with 100 μg/kg of PTH(1-34). Vertebrae were analyzed by μCT. BV/TV, bone volume/tissue volume. Results are expressed as change from vehicle-treated animals, which are ascribed a value of 100%. * p < 0.05, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: The increase in biomechanical properties induced by iPTH treatment is blunted in Naca / Lrp6 compound heterozygotes. Four-month-old female mice were treated once daily, 5 days/week for 1 month with 100 μg/kg of PTH(1-34). L2 vertebrae were analyzed by compression testing to measure stiffness ( A ), load at yield ( B ) and maximum load ( C ). Results are expressed as change from vehicle-treated animals, which are ascribed a value of 100%. * p < 0.05; ** p < 0.01; # p < 0.0001, two-way analysis of variance (ANOVA) with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Gene expression monitoring in control ( Naca 99S/S ; Lrp6 +/+ ;OCN-Cre) and compound heterozygous ( Naca 99S/A ; Lrp6 +/fl ;OCN-Cre) littermates. RNA was extracted from marrow-flushed tibial diaphyses, reverse-transcribed and analyzed by RT-qPCR. Relative expression of Lrp6 ( A ), Alpl ( B ) and Col1a1 ( C ) is shown. * p < 0.05; ** p < 0.01; *** p < 0.001, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques: Gene Expression, Control, Reverse Transcription, Quantitative RT-PCR, Expressing

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Parallel PTH signaling pathways in control ( Naca 99S/S ; Lrp6 +/+ ;OCN-Cre) and compound heterozygous ( Naca 99S/A ; Lrp6 +/fl ;OCN-Cre) littermates. Protein extracts were prepared from tibial shafts and immunoprobed for β-Catenin ( A ) and SIK2 ( B ). Equivalent loading was assessed by probing for α-Tubulin ( C ).

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques: Protein-Protein interactions, Control

Journal: Brain Sciences

Article Title: Expression of WNT Signaling Genes in the Dorsolateral Prefrontal Cortex in Schizophrenia

doi: 10.3390/brainsci14070649

Figure Lengend Snippet: TaqMan primers.

Article Snippet: LRP6 , Low-density lipoprotein receptor-related protein 6 , Hs00233945_m1.

Techniques: Ubiquitin Proteomics

Journal: Brain Sciences

Article Title: Expression of WNT Signaling Genes in the Dorsolateral Prefrontal Cortex in Schizophrenia

doi: 10.3390/brainsci14070649

Figure Lengend Snippet: Relative mRNA expression of WNT signaling pathway genes in the dorsolateral prefrontal cortex (DLPFC) tissue of schizophrenia ( n = 20) compared to non-psychiatrically ill control ( n = 20) subjects, reported as percent of control (geometric mean of reference genes β2M and GAPDH). Data presented as mean ± standard error of the mean (SEM). Abbreviations : IQGAP1, IQ motif containing GTPase activating protein 1; CTNNβ1, Beta-catenin; GSK3β, Glycogen synthase kinase 3 beta; FOXO1, Forkhead box O1; LRP6, Lipoprotein receptor-related protein 6; MGEA5, Meningioma expressed antigen 5; TCF4, Transcription factor 4; βTRC, Beta-transducin repeat containing E3 ubiquitin protein ligase; PPP1Cβ, Protein phosphatase 1 catalytic subunit beta; DVL2, Disheveled segment polarity protein 2.

Article Snippet: LRP6 , Low-density lipoprotein receptor-related protein 6 , Hs00233945_m1.

Techniques: Expressing, Control, Ubiquitin Proteomics

Journal: Brain Sciences

Article Title: Expression of WNT Signaling Genes in the Dorsolateral Prefrontal Cortex in Schizophrenia

doi: 10.3390/brainsci14070649

Figure Lengend Snippet: Heatmap generated from Kaleidoscope “lookup” study assessing mRNA expression of WNT signaling pathway genes across 20 RNA-seq and microarray-derived brain region-level transcriptomic datasets between schizophrenia and control groups. The heatmap utilizes unsupervised hierarchical clustering for both genes and datasets. The data are shown as log 2 -fold change (LFC) values: red indicates increased mRNA expression and blue indicates decreased mRNA expression on a scale of 2 to −2. The x-axis lists the WNT genes queried, and the y-axis lists the Kaleidoscope datasets. Abbreviations : IQGAP1, IQ motif containing GTPase activating protein 1; CTNNβ1, Beta-catenin; GSK3β, Glycogen synthase kinase 3 beta; FOXO1, Forkhead box O1; LRP6, Lipoprotein receptor-related protein 6; TCF4, Transcription factor 4; βTRC, Beta-transducin repeat containing E3 ubiquitin protein ligase; PPP1Cβ, Protein phosphatase 1 catalytic subunit beta; DVL2, Disheveled segment polarity protein 2.

Article Snippet: LRP6 , Low-density lipoprotein receptor-related protein 6 , Hs00233945_m1.

Techniques: Generated, Expressing, RNA Sequencing, Microarray, Derivative Assay, Control, Ubiquitin Proteomics

Journal: Brain Sciences

Article Title: Expression of WNT Signaling Genes in the Dorsolateral Prefrontal Cortex in Schizophrenia

doi: 10.3390/brainsci14070649

Figure Lengend Snippet: Heatmap generated from Kaleidoscope “lookup” study assessing mRNA expression of WNT signaling pathway genes across 15 RNA-seq and microarray-derived cell-level transcriptomic datasets between schizophrenia and control groups. The heatmap utilizes unsupervised hierarchical clustering for both genes and datasets. The data are shown as log 2 -fold change (LFC) values: red indicates increased mRNA expression and blue indicates decreased mRNA expression on a scale of 2 to −2. The x-axis lists the WNT genes queried, and the y-axis lists the Kaleidoscope datasets. Abbreviations : IQGAP1, IQ motif containing GTPase activating protein 1; CTNNβ1, Beta-catenin; GSK3β, Glycogen synthase kinase 3 beta; FOXO1, Forkhead box O1; LRP6, Lipoprotein receptor-related protein 6; TCF4, Transcription factor 4; βTRC, Beta-transducin repeat containing E3 ubiquitin protein ligase; PPP1Cβ, Protein phosphatase 1 catalytic subunit beta; DVL2, Disheveled segment polarity protein 2.

Article Snippet: LRP6 , Low-density lipoprotein receptor-related protein 6 , Hs00233945_m1.

Techniques: Generated, Expressing, RNA Sequencing, Microarray, Derivative Assay, Control, Ubiquitin Proteomics

Journal: Brain Sciences

Article Title: Expression of WNT Signaling Genes in the Dorsolateral Prefrontal Cortex in Schizophrenia

doi: 10.3390/brainsci14070649

Figure Lengend Snippet: Heatmap generated from Kaleidoscope “lookup” study assessing mRNA expression of WNT signaling pathway genes across 15 RNA-seq and microarray rodent-model derived transcriptomic datasets between antipsychotic-treated and vehicle-treated (control) groups. The heatmap utilizes unsupervised hierarchical clustering for both genes and datasets. The data are shown as log 2 -fold change (LFC) values: red indicates increased mRNA expression and blue indicates decreased mRNA expression on a scale of 2 to −2. The x-axis lists the WNT genes queried, and the y-axis lists the Kaleidoscope datasets. Abbreviations : IQGAP1, IQ motif containing GTPase activating protein 1; CTNNβ1, Beta-catenin; GSK3β, Glycogen synthase kinase 3 beta; FOXO1, Forkhead box O1; LRP6, Lipoprotein receptor-related protein 6; TCF4, Transcription factor 4; βTRC, Beta-transducin repeat containing E3 ubiquitin protein ligase; PPP1Cβ, Protein phosphatase 1 catalytic subunit beta; DVL2, Disheveled segment polarity protein 2.

Article Snippet: LRP6 , Low-density lipoprotein receptor-related protein 6 , Hs00233945_m1.

Techniques: Generated, Expressing, RNA Sequencing, Microarray, Derivative Assay, Control, Ubiquitin Proteomics

Journal: Life

Article Title: The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer

doi: 10.3390/life13030615

Figure Lengend Snippet: LRP6 expression in normal colon and CRC tissues. ( A ) LRP6 expression in normal colon mucosa. ( B , C ) Classification of the positivity of LRP6 expression in CRC. ( D , E ) Classification of LRP6 expression intensity in CRC. ( F ) Distribution of the staining score, calculated by multiplying the intensity and positivity scores.

Article Snippet: The product numbers of the TaqMan Gene Expression assay were as follows: ACTB ID Hs01060665_g1 and LRP6 ID Hs00999795_m1.

Techniques: Expressing, Staining

Journal: Life

Article Title: The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer

doi: 10.3390/life13030615

Figure Lengend Snippet: LRP6 and β-catenin expression in normal esophageal and ESCC tissues. ( A ) LRP6 expression in normal esophageal tissues. ( B , C ) Distribution of the LRP6 staining score, calculated by multiplying the intensity and positivity scores. ( D , E ) Distribution of the β-catenin staining score, calculated by multiplying the intensity and positivity scores.

Article Snippet: The product numbers of the TaqMan Gene Expression assay were as follows: ACTB ID Hs01060665_g1 and LRP6 ID Hs00999795_m1.

Techniques: Expressing, Staining

Journal: Life

Article Title: The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer

doi: 10.3390/life13030615

Figure Lengend Snippet: Effects of LRP6 on Wnt/β-catenin signaling activity. ( A ) Expression of LRP6 mRNA in CRC and ESCC cell lines, as measured by qRT-PCR. We used ACTB as endogenous control. * p < 0.01. ( B ) The protein level of LRP6 in CRC and ESCC cell lines. We used ACTB as endogenous control. ( C ) Wnt/β-catenin signaling activity under unstimulated conditions in CRC and ESCC cell lines. ( D ) Experimental plan for investigating signaling activity by adding LRP6-siRNA or Wnt3a to the CRC and ESCC cell lines. ( E – H ) Wnt/β-catenin signaling activity using CRC and ESCC cell lines. We used Renilla as endogenous control. * p < 0.05, ** p < 0.01; n.s.: not significant.

Article Snippet: The product numbers of the TaqMan Gene Expression assay were as follows: ACTB ID Hs01060665_g1 and LRP6 ID Hs00999795_m1.

Techniques: Activity Assay, Expressing, Quantitative RT-PCR, Control

Journal: Life

Article Title: The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer

doi: 10.3390/life13030615

Figure Lengend Snippet: Comparison of clinicopathological status stratified by LRP6 or β-catenin expression in CRC tissue samples.

Article Snippet: The product numbers of the TaqMan Gene Expression assay were as follows: ACTB ID Hs01060665_g1 and LRP6 ID Hs00999795_m1.

Techniques: Comparison, Expressing

Journal: Life

Article Title: The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer

doi: 10.3390/life13030615

Figure Lengend Snippet: Comparison of clinicopathological status stratified by LRP6 or β-catenin expression in ESCC tissue samples.

Article Snippet: The product numbers of the TaqMan Gene Expression assay were as follows: ACTB ID Hs01060665_g1 and LRP6 ID Hs00999795_m1.

Techniques: Comparison, Expressing

Journal: PLoS ONE

Article Title: Effects of pharmacologic sclerostin inhibition or testosterone administration on soleus muscle atrophy in rodents after spinal cord injury

doi: 10.1371/journal.pone.0194440

Figure Lengend Snippet: A-C. LDL receptor related protein 5 (LRP5) and 6 (LRP6), and androgen receptor (AR) gene expressions in tibia, levator ani/bulbocavernosus (LABC), and soleus from animals receiving sham surgery (T 8 laminectomy). Values are means ± SEM [corrected for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the housekeeping gene] and expressed relative to the tibia, n = 6/tissue. Letters a-c indicate differences from respectively labeled groups at p<0.05 or *p<0.01 (a = vs Tibia; b = vs LABC; c = vs Soleus).

Article Snippet: The following commercially-available gene-specific primers were used (ThermoFisher Scientific, Carlsbad, CA): rat LRP5 (#Rn01451428_m1), rat LRP6 (#Rn01492711_m1), rat AR (#Rn00560747_m1), and rat GAPDH (#Rn01775763_m1).

Techniques: Labeling